黄色大片在线观看,国产精品一区二区中文字幕丝袜,国产美女裸体视频永久免费无遮挡,欧美成人精品

咨詢熱線

15000266580

當(dāng)前位置:首頁 >產(chǎn)品中心>細(xì)胞庫>倉鼠肺細(xì)胞>CRL-1657R 1610 倉鼠肺細(xì)胞

R 1610 倉鼠肺細(xì)胞

簡要描述:CRL-1657 R 1610 倉鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-1657
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-08-29
  • 訪  問  量:1869

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

CRL-1657 R 1610 倉鼠肺細(xì)胞,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)優(yōu)培養(yǎng)條件


CRL-1657 R 1610 倉鼠肺細(xì)胞 的詳細(xì)介紹

CRL-1657

ATCC® Number:



Shipped:  frozen CRL-1657™

Designations:  R 1610

Depositors:   M Rosenberg

Biosafety Level: 1

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus (hamster, Chinese)

Morphology: fibroblast



Source: Organ: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host

Gender:  male

Comments: This line is a clonal derivative of V79 cells (see also V79-4, ATCC CCL-93) that had been treated with ethylmethanesulfonate.

The cells are deficient in galactokinase and are resistant to 2-deoxygalactose and 8-azaguanine.

This is a Chinese hamster somatic cell line.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.



Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liqid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

parental cell line:ATCC CCL-93

References: 10111: Schumperli D, et al. Efficient expression of Escherichia coli galactokinase gene in mammalian cells. Proc. Natl. Acad. Sci. USA 79: 257-261, 1982. PubMed: 6281760

25665: Thirion JP, et al. Galactokinase mutants of Chinese hamster somatic cells resistant to 2-deoxygalactose. Genetics 83: 137-147, 1976. PubMed: 1269916

Related Links

NCBI Entrez Search

Make a Deposit

Frequently Asked Questions

Material Transfer Agreement

Technical Support

Related Cell Culture Products




















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸